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. 2012 Feb 16;7(2):e31252. doi: 10.1371/journal.pone.0031252

Figure 1. Determination of curli production by Congo red binding.

Figure 1

Phenotypes on CR medium of MG1655 (wild type strain), AM70 (csgA deletion mutant, unable to produce curli), MG1655carB::Tn5kan, MG1655carB::Tn5kan ΔcsgA::cat and MG1655ΔcarB::cat. Strains were grown either at 30°C (for 24 hours) or at 37°C (for 18 hours). Plates were incubated for 48 hours at 4°C to enhance Congo red binding.