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. 2012 Feb 16;7(2):e31252. doi: 10.1371/journal.pone.0031252

Figure 3. Congo red binding by E. coli strains deficient in UMP biosynthesis.

Figure 3

The MG1655 strain and isogenic mutants deficient in UMP biosynthetic genes were spotted on either CR medium or CR(ura) medium (CR medium supplemented with 0.25 mM uracil) and grown for 24 hours at 30°C. Plates were incubated for 48 hours at 4°C to enhance Congo red binding.