Skip to main content
. 2012 Feb 16;7(2):e31252. doi: 10.1371/journal.pone.0031252

Figure 6. Determination of cellulose amounts.

Figure 6

Strains MG1655, MG1655ΔcarB::cat, and MG1655ΔpyrC::tet were grown 48 hours at 30°C on either LB1/4 agar (no added uracil, Cellulose extraction and determination was performed as described [35]. Data shown are the average of two independent experiments giving very similar results. For strains MG1655ΔcarB::cat and MG1655ΔpyrC::tet grown on LB1/4 agar no glucose was detectable in the assays. A value of 0.5 nmol glucose, corresponding to the lowest detectable concentration in the assay, as determined by a glucose standard curve, was thus arbitrarily assigned to these strains.