Table 2. Escherichia coli strains and plasmids used in this work.

Escherichia coliStrains	Relevant genotype or characteristics	Reference or source
MG1655	K-12, F− λ− rph-1	Standard laboratory strain [52]
AM70	MG1655 ΔcsgA::cat	[37]
LG28	MG1655 ΔbcsA::kan	[35]
LG30	MG1655 ΔadrA::kan obtained by P1 transduction from 3934adrA [38]	This work
MG1655carB::Tn5kan	Tn5::kan transposon inserted at nucleotide 2720 of the carB gene	This work
MG1655ΔcarB::cat	Replacement of the nucleotides 1–550 of the carB gene with a chloramphenicol resistance cassette	This work
MG1655ΔcytR::cat	Replacement of the cytR gene with a chloramphenicol resistance cassette	This work
MG1655ΔpurH::cat	Replacement of the purH gene with a chloramphenicol resistance cassette	This work
MG1655ΔpyrB::cat	Replacement of the pyrB gene with a chloramphenicol resistance cassette	This work
MG1655ΔpyrC::tet	Replacement of the pyrC gene with a tetracycline resistance cassette	This work
MG1655ΔpyrE::tet	Replacement of the pyrE gene with a tetracycline resistance cassette	This work
MG1655ΔrutR::cat	Replacement of the rutR gene with a chloramphenicol resistance cassette	This work
MG1655ΔyedQ::kan	Replacement of the yedQ gene with a kanamycin cassette	This work
MG1655carB::Tn5kan ΔcsgA::cat	Obtained by P1 transduction from AM70 into MG1655carB::Tn5kan	This work
MG1655ΔcarB::cat ΔbcsA::kan	Obtained by P1 transduction from LG28 into MG1655ΔcarB::cat	This work
MG1655ΔcarB::cat ΔadrA::kan	Obtained by P1 transduction from LG30 into MG1655ΔcarB::cat	This work
MG1655ΔcarB::cat ΔyedQ::kan	Obtained by inactivation of the yedQ gene by λ red technique	This work
MG1655ΔpyrB::cat ΔbcsA::kan	Obtained by P1 transduction from LG28 into MG1655ΔpyrB::cat	This work
MG1655ΔpyrB::cat ΔadrA::kan	Obtained by P1 transduction from LG30 into MG1655ΔpyrB::cat	This work
MG1655ΔpyrB::cat ΔyedQ::kan	Obtained by inactivation of the yedQ gene by λ red technique	This work
Plasmids
pCR2.1	Control vector allowing direct cloning of PCR products, ampicillin resistance	Invitrogen
pCR2.1-carB	carB gene cloned as PCR product into pCR2.1 vector	This work