MG1655 |
K-12, F− λ−
rph-1
|
Standard laboratory strain [52]
|
AM70 |
MG1655 ΔcsgA::cat
|
[37]
|
LG28 |
MG1655 ΔbcsA::kan
|
[35]
|
LG30 |
MG1655 ΔadrA::kan obtained by P1 transduction from 3934adrA
[38]
|
This work |
MG1655carB::Tn5kan
|
Tn5::kan transposon inserted at nucleotide 2720 of the carB gene |
This work |
MG1655ΔcarB::cat
|
Replacement of the nucleotides 1–550 of the carB gene with a chloramphenicol resistance cassette |
This work |
MG1655ΔcytR::cat
|
Replacement of the cytR gene with a chloramphenicol resistance cassette |
This work |
MG1655ΔpurH::cat
|
Replacement of the purH gene with a chloramphenicol resistance cassette |
This work |
MG1655ΔpyrB::cat
|
Replacement of the pyrB gene with a chloramphenicol resistance cassette |
This work |
MG1655ΔpyrC::tet
|
Replacement of the pyrC gene with a tetracycline resistance cassette |
This work |
MG1655ΔpyrE::tet
|
Replacement of the pyrE gene with a tetracycline resistance cassette |
This work |
MG1655ΔrutR::cat
|
Replacement of the rutR gene with a chloramphenicol resistance cassette |
This work |
MG1655ΔyedQ::kan
|
Replacement of the yedQ gene with a kanamycin cassette |
This work |
MG1655carB::Tn5kan ΔcsgA::cat
|
Obtained by P1 transduction from AM70 into MG1655carB::Tn5kan
|
This work |
MG1655ΔcarB::cat ΔbcsA::kan
|
Obtained by P1 transduction from LG28 into MG1655ΔcarB::cat
|
This work |
MG1655ΔcarB::cat ΔadrA::kan
|
Obtained by P1 transduction from LG30 into MG1655ΔcarB::cat
|
This work |
MG1655ΔcarB::cat ΔyedQ::kan
|
Obtained by inactivation of the yedQ gene by λ red technique |
This work |
MG1655ΔpyrB::cat ΔbcsA::kan
|
Obtained by P1 transduction from LG28 into MG1655ΔpyrB::cat
|
This work |
MG1655ΔpyrB::cat ΔadrA::kan
|
Obtained by P1 transduction from LG30 into MG1655ΔpyrB::cat
|
This work |
MG1655ΔpyrB::cat ΔyedQ::kan
|
Obtained by inactivation of the yedQ gene by λ red technique |
This work |
Plasmids
|
pCR2.1 |
Control vector allowing direct cloning of PCR products, ampicillin resistance |
Invitrogen |
pCR2.1-carB
|
carB gene cloned as PCR product into pCR2.1 vector |
This work |