Figure 3. Relative ID errors at 30 arbitrary distance thresholds.

Relative ID errors (FP/n. of not discarded queries) obtained from a library of 602 tephritid DNA barcodes. Linear fitting (95% confidence intervals are indicated) was used to infer an ad hoc distance threshold corresponding to a relative ID error <0.05 (THR_{K2P_0.05}). Error estimates were verified through the DNA barcoding identification of an independent set of 188 tephritid specimens. The grey area represents the relative ID error (estimated and observed) corresponding to THR_{K2P_0.05}.