Figure 3. High avidity T cells expand preferentially after adoptive transfer into Cy and vaccine-treated neu-N mice.

Mice were injected with 1×10⁶ tumor cells/mouse one week prior to Cy and vaccine treatment. High avidity or low avidity T cells were adoptively transferred one day after vaccination. (A) The absolute # of Thy1.2 T cells were calculated in the tumor draining lymph nodes (TDNs) of neu-N mice on day 3 (White bars), 5 (Gray bars), and 8 (Black bars) after adoptive transfer (n = 9 mice per group). (B) The absolute # of Thy1.2 T cells were calculated in the vaccine draining lymph nodes (VDNs) on day 3 (White bars), 5 (Gray bars), and 8 (Black bars) after adoptive transfer (n = 9 mice per group). Treatment groups are as indicated. High/low = High or low avidity T cells, respectively. Cy = Cytoxan treated. Vaccine = 3T3neuGM vaccine, Mock = 3T3GM control vaccine. Each experiment was repeated three times with similar results.