Figure 6. Cy-mediated Treg depletion increases high avidity T cell tumor trafficking in neu-N mice.

Neu-N mice were treated as in Figure 3 . (A and B) On day 3 (White bars) and day 5 (Gray bars) after adoptive transfer, the absolute number of Thy1.2⁺ cells/mg of tumor was calculated for each treatment group (n = 3 mice/group). (C) On day 5 after adoptive transfer the ratio of Thy1.2⁺ cells/CD4⁺Foxp3⁺ cells was calculated (n = 3 mice/group). (D) Lymphocytes from tumors on day 5 after adoptive transfer were stimulated as described in the methods and the %Thy1.2⁺ cells producing IFNγ after RNEU_420–429 stimulation was calculated after NP stimulation was subtracted (n = 5mice/group). * = p<0.05. High/low = High or low avidity T cell transfer, respectively. Cy = Cyclophosphamide treatment. Vaccine = 3T3neuGM vaccine, Mock = 3T3GM control vaccine. Each experiment was repeated three times with similar results.