Table 1. Primers used in this study.
Primer | Sequence (5′-3′) |
Clone primers | |
P1 (oligo (dT)-adaptor) | GGCCACGCGTCGACTAGTACT 17 |
P2 (CfCSP primer) | TAGGAAAGAAACACCAACTCACTCG |
P3 (T7) | GTAATACGACTCACTATAGGGC |
Sequencing primers | |
P4 (M13-47) | CGCCAGGGTTTTCCCAGTCACGAC |
P5 (RV-M) | GAGCGGATAACAATTTCACACAGG |
RT primers | |
P6 (CfCSP-RTF) | GACTGCCATTACAAAGAACAACCC |
P7 (CfCSP-RTR) | GCCAGGTCCTCCTCGGTAGTAA |
P8 (β-actin-RTF) | CAAACAGCAGCCTCCTCGTCAT |
P9 (β-actin-RTR) | CTGGGCACCTGAACCTTTCGTT |
Transcription primers | |
P10 (Luc-mRNAF) | GCGTAATACGACTCACTATAGGATGGAAGACGCCAAAAACAT |
P11 (Luc-mRNAR) | TTACACGGCGATCTTTCCGC |
Recombination primers | |
P12 (CfCSP-ReF1) | TTAGAGCTCGGACAAATGGTAACACCTTTATTA |
P13 (CfCSP-ReR1) | AGAAAGCTTCCAGCGAGAGATGCTTACT |
P14 (CfCSP-ReF2) | TTACATATGGGACAAATGGTAACACCTTTATTA |
P15 (CfCSP-ReR2) | AGAGAATTCCCCAGCGAGAGATGCTTACT |
P16 (CSPA-ReF) | TTACATATGATGTCCGGTAAAATGACTGGTATC |
P17 (CSPA-ReR) | AGAGAATTCCAGGCTGGTTACGTTACCAGCT |