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. 2012 Feb 16;7(2):e32012. doi: 10.1371/journal.pone.0032012

Table 1. Primers used in this study.

Primer Sequence (5′-3′)
Clone primers
P1 (oligo (dT)-adaptor) GGCCACGCGTCGACTAGTACT 17
P2 (CfCSP primer) TAGGAAAGAAACACCAACTCACTCG
P3 (T7) GTAATACGACTCACTATAGGGC
Sequencing primers
P4 (M13-47) CGCCAGGGTTTTCCCAGTCACGAC
P5 (RV-M) GAGCGGATAACAATTTCACACAGG
RT primers
P6 (CfCSP-RTF) GACTGCCATTACAAAGAACAACCC
P7 (CfCSP-RTR) GCCAGGTCCTCCTCGGTAGTAA
P8 (β-actin-RTF) CAAACAGCAGCCTCCTCGTCAT
P9 (β-actin-RTR) CTGGGCACCTGAACCTTTCGTT
Transcription primers
P10 (Luc-mRNAF) GCGTAATACGACTCACTATAGGATGGAAGACGCCAAAAACAT
P11 (Luc-mRNAR) TTACACGGCGATCTTTCCGC
Recombination primers
P12 (CfCSP-ReF1) TTAGAGCTCGGACAAATGGTAACACCTTTATTA
P13 (CfCSP-ReR1) AGAAAGCTTCCAGCGAGAGATGCTTACT
P14 (CfCSP-ReF2) TTACATATGGGACAAATGGTAACACCTTTATTA
P15 (CfCSP-ReR2) AGAGAATTCCCCAGCGAGAGATGCTTACT
P16 (CSPA-ReF) TTACATATGATGTCCGGTAAAATGACTGGTATC
P17 (CSPA-ReR) AGAGAATTCCAGGCTGGTTACGTTACCAGCT