Table 1.
Species quantification results from a survey of selected xenografts.
| Xenograft Name | Tumor Type |
in vivo Passage | Initial DNA (ng) | Average % Human ± SD | Average % Mouse |
Calculated Human DNA |
Calculated Mouse DNA |
Sum DNA (ng) | % of Initial DNA |
|---|---|---|---|---|---|---|---|---|---|
| HL-60(TB) | Leukemia | P01 | 27.6 | 100.0 ± 0.00 | 0.0 | 26.96 | 0.00 | 27.0 | 97.7 |
| MDA-MB-361 | Breast | P01 | 48.8 | 12.8 ± 1.84 | 87.2 | 4.80 | 31.20 | 36.0 | 73.7 |
| NCI-H157 | Lung | P01 | 72.4 | 98.9 ± 1.50 | 1.1 | 97.83 | 1.29 | 99.1 | 136.8 |
| RXF-631 | Renal | P01 | 23.2 | 43.2 ± 0.06 | 56.8 | 9.90 | 11.34 | 21.2 | 91.5 |
| SK-MEL-2 | Melanoma | P01 | 29.2 | 81.8 ± 1.42 | 18.2 | 38.63 | 7.66 | 46.3 | 158.4 |
| UACC-257 | Melanoma | P01 | 53.7 | 99.9 ± 0.08 | 0.1 | 49.49 | 0.04 | 49.5 | 92.3 |
| LOX IMVI | Melanoma | P01-a | 50.6 | 48.4 ± 7.26 | 51.6 | 16.15 | 17.54 | 33.7 | 66.6 |
| LOX IMVI | Melanoma | P01-b | 52.8 | 55.1 ± 6.38 | 45.0 | 19.30 | 16.77 | 36.1 | 68.3 |
| LOX IMVI | Melanoma | P01-c | 55.5 | 39.6 ± 7.99 | 60.4 | 17.13 | 27.34 | 44.5 | 80.2 |
| LOX IMVI | Melanoma | P01-d | 52.1 | 53.1 ± 6.56 | 46.9 | 24.81 | 35.35 | 60.2 | 115.4 |
| LOX IMVI | Melanoma | P01-e | 53.5 | 33.9 ± 7.76 | 66.1 | 20.15 | 45.56 | 65.7 | 122.9 |
| AS283 | Lymphoma | P04-a | 56.9 | 83.5 ± 5.89 | 16.5 | 37.12 | 7.39 | 44.5 | 78.3 |
| AS283 | Lymphoma | P04-b | 71.6 | 83.6 ± 4.90 | 16.4 | 86.85 | 13.31 | 100.2 | 139.8 |
| CA46 | Lymphoma | P04 | 43.4 | 99.9 ± 0.09 | 0.1 | 47.76 | 0.04 | 47.8 | 110.2 |
| CP70 | Ovarian | P04 | 61.4 | 83.5 ± 3.23 | 16.6 | 59.56 | 9.87 | 69.4 | 113.0 |
| Daudi | Lymphoma | P04 | 56.5 | 100.0 ± 0.02 | 0.0 | 49.03 | 0.01 | 49.0 | 86.7 |
| GTL-16 | Gastric | P04 | 42.3 | 100.0 ± 0.00 | 0.0 | 39.92 | 0.00 | 39.9 | 94.4 |
| OVCAR-5 | Ovarian | P04 | 53 | 88.8 ± 0.31 | 11.2 | 63.11 | 6.98 | 70.1 | 132.1 |
| AS283 | Lymphoma | P10 | 49.2 | 99.3 ± 0.82 | 0.7 | 48.04 | 0.22 | 48.3 | 98.1 |
| HuH-7 | Liver | P10 | 55.2 | 90.1 ± 4.26 | 9.9 | 52.24 | 4.36 | 56.6 | 102.5 |
| NCI-H1975-Luc-GFP | Lung | P10 | 56.4 | 34.9 ± 6.18 | 65.1 | 18.67 | 29.45 | 48.1 | 85.4 |
| NCI-H209-Luc-GFP | Lung | P10 | 61.7 | 81.0 ± 2.00 | 19.0 | 79.70 | 15.83 | 95.5 | 154.8 |
The xenograft names, tumor types, and number of in vivo passages a sample has progressed through are given for each sample at the time of sample collection. Letters following the passage number signify independent tumor samples derived from tumors grown on different mice of the same cohort. After DNA extraction and purification, samples were diluted between 25 ng/μl and 75 ng/μl; one microliter of this dilution serves as the initial DNA (ng) template in the qPCR reactions. The CT value for each sample was derived from standard curves and converted to a log genomes value, which can be converted to the number of measured haploid genomes present. Percent Human and Percent Mouse values were derived by dividing # human genomes by total genomes present or # mouse genomes divided by total genomes present, respectively. Genome numbers for each species are back-calculated into DNA (ng), the sum of which should be equal to the initial DNA used in the qPCR sample. The far right column lists the percentage of the initial sample calculated from the qPCR assay and gives a measure of quality assurance.