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. 2012 Feb 16;7(2):e32211. doi: 10.1371/journal.pone.0032211

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Stark et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were infected with LM-OVA and challenged with B16-OVA subcutaneously 21 days (A), 93 days (B) or 126 days (C) post-infection (dpi). Tumor injection time point is indicated by an arrow on the x axis. The mean ± SD (n = 5 per group) of the frequency of OVA_257–264-specific CD8⁺ T cells in the blood at various times is indicated (A, B, C). The frequency blood OVA_257–264-specific CD8⁺ T cells on day 7 and 21 after LM-OVA infection in individual animals from seven different experiments is presented (D & E). Representative profile of blood OVA_257–264-specific CD8⁺ T cell percentages (F). Response on day 7 is statistically different between the two mouse strains by two tailed, unpaired t test with Welsh's correction for unequal variance; **, P<0.001, ***, P<0.0001.