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. 2012 Feb 16;7(2):e32211. doi: 10.1371/journal.pone.0032211

Figure 5. Antigen presentation and proliferation.

Figure 5

Mean ± SD of triplicate cultures of CFSE reduction correlates to proliferation of OT.1 CD8+ T cells in response to DCs from LM or LM-OVA infected mice. Positive controls were pulsed with OVA257–264 peptide in vitro. Data are significantly different, ***(p<0.0001), relative to negative control (LM infected DCs) by two tailed t test with Welch's correction for unequal variance. Data are representative of 2 independent DC isolation experiments. (B) Representative flow cytometric profile indicating in-vitro antigen-dependent proliferation of OVA257–264-specific CD8+ T cells on different days of in vitro culture. Samples were obtained from in-vitro cultures in T-75 flasks. Data are representative of three such independent experiments conducted.