Figure 5.

Effect of thermal therapy on p53, Bax and Bcl-2 detected by Western blotting. Lovo cells were treated with 12.5 μg/mL or 50 μg/mL oxaliplatin for 1 h at 37  °C, 39  °C, 41  °C, 43  °C or 45  °C. Cells were harvested, and total proteins were extracted and immunoblotted for p53, Bax and Bcl-2. The values represent means ± SD of at least three separate experiments. Beta-actin was used as loading control (data not shown).