Table 3. List of 12 functions that evaluate binding strength of the core region (P6–P2′) and solvent accessibility of two flanking regions (P7–P14 and P3′–P6′).

functions	Physical property	Position on the furin cleavage site motif	Description8
	ZIMJ68010425	P2 P4 P5 P6	Positive charge and isoelectric point
	ZIMJ68010425	P2 P3	Positive charge and isoelectric point
	Cysteine8	P2–P6	Disulfide bond formation potential and negative charge
	ZIMJ68010425, EISD84010114, KUHL95010126	P4	Aliphatic residue or positively charged residue
	FAUJ88011127	P4–P6	Positive charge compensation
	BULH74010228	P1′	Volume
	BULH74010228	P1′ – P3′	Volume
	KARP85010329	P1′ P2 P4 P5 P6	Flexibility
	KARP85010329	P1′ – P3′	Flexibility
	EISD84010114	P7–P10	Hydrophobicity
	EISD84010114	P3′–P6′	Hydrophobicity
	EISD84010114	P11–P14	Hydrophobicity