Ex vivo Expansion increases NKG2D-dependent tumor cell lysis via NKG2D+CD8+ T Cell degranulation. A demonstrates increased tumor cell lysis by NKG2D+CD8+ T cells after 7 days of ex vivo expansion. P values represent statistical significance when comparing specific lysis of target cells by NKG2D+CD8+ T cells at baseline and at day 7 of expansion. Targets include a CML cell line (K562) and a Myeloma cell line (RPMI8226). B demonstrates that, along with an increase in tumor cell lysis, there is a significant increase in CD107a expression, suggesting degranulation of NKG2D+CD8+ T cells. These results indicate that, along with lysis of tumor targets, NKG2D+CD8+ T cells’ degranulation induces apoptosis in tumor cells. P values represent statistical significance when comparing degranulation in NKG2D+CD8+ T cells at baseline and after 7 days of expansion.