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. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Biomaterials. 2011 Oct 2;33(1):29–37. doi: 10.1016/j.biomaterials.2011.09.044

Levels of secondary necrosis present in monocyte and PMN cultures as determined by measuring the activity of caspase-3 in the supernatant. Supernatant from cells exposed to TCPS () and PEG hydrogel () substrates for 2, 24, 48, 96, and 168hr were exposed to fluorogenic caspase-3 substrate and the mean fluorescence intensity from the cleaved probe was assessed.

Inline graphic — Levels of secondary necrosis present in monocyte and PMN cultures as determined by measuring the activity of caspase-3 in the supernatant. Supernatant from cells exposed to TCPS () and PEG hydrogel () substrates for 2, 24, 48, 96, and 168hr were exposed to fluorogenic caspase-3 substrate and the mean fluorescence intensity from the cleaved probe was assessed.