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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Mar 31;1809(4-6):276–283. doi: 10.1016/j.bbagrm.2011.02.002

Figure 6. p150Sal2 binds to the BAX promoter and upregulates its expression.

Figure 6

A - Schematic of the human BAX promoter showing GC boxes. B thru D - HOSE cells transfected with pcDNA-p150Sal2 or empty vector for 48 hours. B – ChIP assays for p150Sal2 binding to the BAX promoter. Assays were performed on chromatin fragments using antibody to p150Sal2 and normalized to preimmune rabbit IgG. Immunoprecipitated fractions were assayed by real time PCR for binding to the BAX and Aldolase promoters. Primers for BAX flanked the GC box at -117. C – Real time PCR on total cell RNA for BAX and Aldolase mRNAs. Results are normalized to Aldolase. D –Immunblots showing increased level of BAX protein in response to p150Sal2. E thru G - HOSE cells were treated with 25 ug/ml etoposide for 22 hours to induce apoptosis. E - ChIP assay showing increased binding of endogenous p150Sal2 to the BAX promoter in treated cells. F - Real time PCR on total cell RNA showing elevated levels of p150Sal2 and BAX mRNAs in apoptotic cells. G –Immunoblots showing elevated BAX protein in treated cells.