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The full-length fragment of Ostrinia furnacalis P450 was obtained by RT-PCR and RACE technology. (a) The gradient PCR amplification of middle fragment of CYP6AE25 gene by degenerate primers. Lane 1 to Lane 8 represents different annealing temperature viz., 48.0° C, 48.8° C, 50.3° C, 52.4° C, 55.3° C, 57.6° C, 59.1° C, and 60.0° C. (b) The 5′ RACE result of CYP6AE25 gene. (c) The 3′ RACE result of CYP6AE25 gene. High quality figures are available online.
