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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: Biochemistry. 2011 Jul 18;50(32):7057–7066. doi: 10.1021/bi200614y

Figure 4.

Figure 4

Effect of glutathionylation on monomer thermal stability. (a) Representative CD spectra of SOD1 before and after cooling shows reversible decrease in ellipticity at 230 nm. (b) Representative curves for thermally induced unfolding of unmodified (closed symbols) and glutathionylated (open symbols) wild type and mutant SOD1 monitored by circular dichroism at 230 nm. (c) Apparent Tm (Tm*) values obtained by fitting blank-corrected thermal melting data as described in Materials and Methods. Experiments were performed at least in duplicate; Tm* values are reported as the mean of all experiments ± SD.