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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Nat Chem Biol. 2012 Feb 12;8(3):311–317. doi: 10.1038/nchembio.790

Fig. 5. Proline supplementation prevents activation of the AAR by HF.

Fig. 5

A) MEFs were treated with the indicated concentration of inhibitor in the presence or absence of 2 mM proline for 2 hours and assayed by Western blot for total GCN2 and GCN2 phosphorylated on Thr898 using a phospho-specific antibody (Cell Signaling). Data are representative of three separate experiments. B) MEFs were treated with 50 nM HF with or without 2 mM proline, lysed 6 hours later and analyzed by Western blot for expression of the AAR response marker CHOP. Cytoplasmic actin (cActin) is shown as a loading control.