Fig. 4.

qPCR analysis of mRNA in the hepatoblast isolated from E16.5 TgCYP3A4/7-hPXR. Expression of CYP3A, hPXR, and murine GR in hepatoblast was determined after 3 d of treatment with 0.1% DMSO and 1, 10, and 100 μm DHEA. Cells were harvested on the third day for analysis of mRNA by the ΔΔCt method, using 18S as an internal control. The relative fold expression level at different developmental stages was expressed as mean ± sd; n = 4. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with cell treated with 0.1% DMSO.