Table 1.

Primer sequences

Primer list for qPCR	Sequence (5′–3′)
CYP3A7
Forward	AAG TCT GGG GTA TTT ATG ACT
Reverse	CGC TGG TGA ATG TTG GAG AC
CYP3A4
Forward	GCC TGG TGC TCC TCT ATC TA
Reverse	GGC TGT TGA CCA TCA TAA AAG
Cyp3a11
Forward	AGC AGG GAT GGA CCT GG
Reverse	CGG TAG AGG AGC ACC AA
Cyp3a13
Forward	TCC CAG AAT TAC AAA ACC AAA GA
Reverse	TGT GGT CTC ATA GCC AGC AA
Gr
Forward	GGC AAA GGC GAT ACC AGG AT
Reverse	TCC AAC CCA GGG CAA ATG
mPxr
Forward	AGA AGC AGA CTC TGC CTT G
Reverse	ATC TCG CAG GTT CCC TTG CGG A
hPXR
Forward	GGC CAC TGG CTA TCA CTT CAA
Reverse	TTC ATG GCC CTC CTG AAA A
18S
Forward	ATT GGA GCT GGA ATT ACC GC
Reverse	CGG CTA CCA CAT CCA AGG AA
β-Actin
Forward	TAT TGG CAA CGA GCG GTT CC
Reverse	GGC ATA GAG GTC TTT ACG GAT GTC
Primer list for plasmid constructs
p3A7/+103b reverse	AAAAATTAATTAACACTACTTTCCTTCCTTATCTCTCTCCTCTGAGTC
p3A7-9.8kb forward	AAAAAGGCGCGCCTCAGGATGATGCAGGCCTCAGAAA
p3A7-7.8kb forward	AAAAAGGCGCGCCTCCTCTTGGAACTTCATGCCCGAT
p3A7-5.4kb forward	AAAAAGGCGCGCCTGGCTTCATGGCTTAGTCACGTCT
p3A7-3.2kb	AAAAAGGCGCGCCTCACTTGGCCACTGGAAGTCAGAA
GRE-6.2kb forward	AAAAAGGCGCGCCAGAACAGCTTGTGTGGACAAATGGAGGTCAGTGAGTGGTGTGT
GRE-4.9kb forward	AAAAAGGCGCGCCTGTCCCAGCATATGGGACAAATGGAGGTCAGTGAGTGGTGTGT
GRE-3.8kb forward	AAAAAGGCGCGCCAGTACATGTCCAAATGGAGGTCAGTGAGTGGTGTGT
GRE-1.4kb forward	AAAAAGGCGCGCCTGAACAAGCAAAAATGGAGGTCAGTGAGTGGTGTGT
pGL4.10-M
Forward	CTGGCGCGCCGAGCTCACGCGTGCTAGCTTAATTAAC
Reverse	TCGAGTTAATTAAGCTAGCACGCGTGAGCTCGGCGCGCCAGGTAC