FIGURE 3.

NADPH oxidase inhibitor protects Abca4^−/−Rdh8^−/− mouse photoreceptors from light-induced degeneration. A, 4- to 5-week-old Abca4^−/−Rdh8^−/− mice were exposed to white light at 10,000 lux for 30 min after pretreatment with either vehicle control DMSO or APO. B, Abca4^−/−Rdh8^−/− mice were exposed to white light at 10,000 lux for 30 min after pretreatment with either vehicle control (DMSO) or another NADPH oxidase inhibitor, DPI. For both APO and DPI pretreatment, evaluations performed 7 days after illumination included OCT imaging (a, * indicates disrupted photoreceptors in the retinal structure), retinal histological examination (b, * indicates disrupted and decreased length of outer and inner photoreceptor segments; 63×), photoreceptor immunohistochemistry (c, rhodopsin in red, peanut agglutinin in green, and DAPI in blue; 20×) and measurements of outer nuclear layer thickness after DAPI staining (d, RPE, retinal pigmented epithelium; OS, outer segment; IS, inner segment; ONH, optic nerve head).