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Analysis of the importance of phosphosites in AHA2 for the activity of the pump by functional complementation of yeast pma1. The yeast strain RS-72 is dependent on the activity of the H+-ATPase expressed when grown on glucose media. When grown on galactose the endogenous yeast H+-ATPase PMA1 is expressed. A, shown is an analysis of pumps mutated in C-terminal phosphosites. B, shown is an analysis of pumps mutated in the N-terminal phosphosites.
