TABLE 1.
Phosphorylation sites in AHAs expressed homologously in plant membranes
| Phosphorylation sites detected in AHA isoforms | Residue in AHA2a | Cytosolic domain | Conservation between AHAsb | Reference |
|---|---|---|---|---|
| Ser-2-AHA1 | Ser-2 | N terminus | This work | |
| Ser-3-AHA2 | Ser-3 | N terminus | This work | |
| Ser-3-AHA3 | ||||
| Ser-544-AHA1 | Ala-544 | P domain | 2/11 | This work |
| Thr-881-AHA1 | Thr-881 | C terminus | 10 (11)/11; Ser or Thr | (77, 78) and this work |
| Thr-881-AHA2 | This work | |||
| Thr-889-AHA3 | Lys-888 | C terminus | 1/11; | This work |
| Ser-899-AHA1 | Ser-899 | C terminus | 5 (7)/11; Ser or Thr | (57, 77) and this work |
| Ser-899-AHA2 | This work | |||
| Not detected in this work | Ser-904 | C terminus | 9/11 | (57) |
| Ser-931-AHA2/3/5/6/8/9c | Ser-931 | C terminus | 11/11 | This work |
| Ser-944-AHA2 | Ser-944 | C terminus | 1/11 | (79) and this work |
| Tyr-946-AHA2 | Tyr-946 | C terminus | 10/11 | (56) and this work |
| Thr-948-AHA1 | Thr-947 | C terminus | 11/11 | (44, 56, 57, 77, 78, 80) and this work |
| Thr-947-AHA2 | ||||
| Thr-948-AHA3 | ||||
| Thr-959/955-AHA4/11c |
a Homologous residues in A. thaliana AHA2.
b Number of sequences in which the residue is conserved out of the 11 AHAs encoded for in the genome of A. thaliana (81).
c The identified phosphopeptide cannot be distinguished between several isoforms (AHA2/3/5/6/8 and AHA4/11).