FIGURE 1.

Basal cardiac contractility, isoprenaline-response or Frank-Starling response are not affected by neuronostatin treatment. Effect of NST was studied in isolated rat heart preparation as described under “Experimental Procedures.” A, rat hearts were perfused in Langendorff model with either vehicle or NST (0.1, 1, or 10 nm) for 15 min. B, LV Frank-Starling response was analyzed by stepwise increasing the volume of the intraventricular balloon in Langendorff-perfused hearts treated with either vehicle or NST (10 nm). C, rat hearts were perfused in Langendorff model with either vehicle, ISO, or ISO + NST (10 nm). DT indicates developed tension, DP indicates developed pressure. A and C, results are expressed as a percent change versus baseline values. Data are mean ± S.E. (n = 6–8 for each group). ***, p < 0.001 versus vehicle.