FIGURE 4.

Neuronostatin phosphorylates p38 MAPK in neonatal rat cardiomyocytes. A, cultured NRVMs were treated with vehicle (Ctrl), 0.1 nm NST, or 0.1 nm SST for times shown in the figure. Cell lysates were then immunoblotted for phosphorylated p38 MAPK. B, NRVMs were treated with 0.1 nm NST for times shown in the figure and at the end of the experiment protein samples were collected. Shown are Western blot analyses of phosphorylation of Akt and extracellular signal-regulated kinase (ERK). GAPDH was used as a loading control.