FIGURE 6.

Neuronostatin regulates protein synthesis machinery. A, NRVMs were treated with 0.1 nm NST or vehicle (Ctrl) for times indicated in the figure. Cell lysates were then immunoblotted for phosphorylated eukaryotic translation elongation factor 2 (eEF2). B, cells were treated as in A, and cell lysates were then immunoblotted for phosphorylated eEF2 kinase (eEF2k). C, NRVMs were treated with insulin-like growth factor-1 (IGF-1), insulin (Ins), or ET-1 alone, or in combination with 0.1 nm NST. Shown is analysis of incorporation of radioactive leucine after 24 h of treatment. D, NRVMs were treated with 0.1 nm NST versus vehicle for times indicated in the figure, and cell lysates were then immunoblotted for phosphorylated ACC. *, p < 0.05 versus control; ***, p < 0.001 versus control.