FIGURE 3.

T. gondii fatty acid elongation pathway is localized to the parasite endoplasmic reticulum. Transgenic parasite lines were allowed to infect coverslip cultures and were fixed and processed for immunofluorescence assay 24 h later. Transgenic proteins were marked with an HA (A–C) or a Myc (D–F) epitope tag detected with suitable antibodies (red channel). All strains carried a Der1GFP marker (green) previously shown to localize to the endoplasmic reticulum (17). DAPI and phase contrast images are shown for comparison. See supplemental Table S1 for reference to T. gondii gene models and “Experimental Procedures” for details on gene identification and cloning. Note that all six candidate fatty acid elongation enzymes are found to be associated with the endoplasmic reticulum. Tagged ELO-C produces a more restricted pattern at the apical side of the nuclear envelope.