FIGURE 8.

GC-MS analysis of fatty acid methyl esters from S. cerevisiae complemented with T. gondii ELO-B. Fatty acids from yeast Δelo2/Δelo3 double deletion strain complemented by TgELO-B (see Fig. 5) were extracted and subjected to GC-MS analysis as described under “Experimental Procedures.” An overlay of the chromatogram of fatty acid methyl esters from this strain and the wild type is shown. A, enlargement of the region of the trace showing C22 and C24 methyl esters. B, region showing C26 methyl esters.