TABLE 1.
Percent labeling of fatty acids derived from [U-13C]glucose-fed tachyzoites and HFF
Wild type RH parasites were used to infect HFF and labeled in situ with [13C]glucose. Uninfected HFF were labeled with lsqb]13C]glucose under identical conditions. Percent labeling was determined by GC-MS analysis.
| Cell type | RH | RH | HFF |
|---|---|---|---|
| +/− ATc | − | + | − |
| C14:0 | 87.73 (± 3.86) | 84.68 (± 0.50) | ND |
| C16:0 | 61.56 (± 0.58) | 59.01 (± 1.09) | 0.00 (± 0.00) |
| C18:0 | 12.60 (± 0.55) | 12.64 (± 0.73) | 0.00 (± 0.00) |
| C20:0 | 23.64 (± 12.81) | 16.84 (± 6.41) | 0.00 (± 0.00) |
| C22:0 | 1.14 (± 2.95) | 4.23 (± 1.05) | 0.00 (± 0.00) |
| C24:0 | 14.57 (± 4.41) | 15.38 (± 1.53) | 0.00 (± 0.00) |
| C16:1 | 65.15 (± 1.62) | 63.76 (± 0.47) | 0.00 (± 0.00) |
| C18:1 | 57.97 (± 3.88) | 54.59 (± 0.15) | 1.76 (± 1.77) |
| C20:1 | 70.41 (± 0.82) | 68.62 (± 0.71) | 0.00 (± 0.00) |
| C22:1 | 6.81 (± 8.79) | 9.76 (± 3.44) | 0.00 (± 0.00) |
| C24:1 | 1.31 (± 1.87) | 0.00 (± 2.58) | 0.00 (± 0.00) |
| C26:1 | 82.83 (± 4.14) | 80.37 (± 2.75) | ND |