TABLE 2.
Percent labeling of fatty acids derived from [U-13C]glucose-fed tachyzoites
Mutant parasites were used to infect HFF and labeled in situ with [13C]glucose in the presence or absence of ATc. Percent labeling was determined by GC-MS analysis. Fatty acids for which significant changes in labeling were observed in the presence and absence of ATc using the Wilcoxon rank sum test (p values less than 0.05) are indicated by asterisk.
| Cell type | ACP | ELOA | ELOA | ELOB | ELOB | ELOC | ELOC | |
|---|---|---|---|---|---|---|---|---|
| +/−ATc | + | − | + | − | + | − | + | |
| C14:0 | 54.08* (± 0.99) | 7.66* (± 1.78) | ND | ND | ND | ND | ND | ND |
| C16:0 | 33.58* (± 0.68) | 6.06* (± 0.22) | 45.17 (± 0.64) | 44.24 (±0.19) | 50.01 (± 0.45) | 46.34 (± 1.47) | 45.65 (± 1.28) | 45.14 (± 0.35) |
| C18:0 | 2.38* (± 0.51) | 0.26* (± 0.28) | 10.02* (± 0.50) | 3.08* (± 0.03) | 8.40 (± 0.40) | 10.18 (± 0.54) | 5.95 (± 0.53) | 7.02 (± 0.18) |
| C20:0 | 0.00 (±0.00) | 0.00 (± 0.00) | 17.79* (± 3.14) | 2.28* (± 1.60) | 0.00 (± 0.00) | 20.99 (± 8.83) | 0.00 (± 3.65) | 1.97 (± 7.81) |
| C22:0 | 0.00 (± 0.00) | 0.00 (± 0.39) | 9.27 (± 1.61) | ND | 1.26 (± 2.64) | 4.62 (± 3.55) | 0.00 (± 2.47) | 0.00 (± 0.00) |
| C24:0 | 0.00 (± 4.82) | 1.23 (± 0.08) | 10.21* (± 1.47) | 3.75*(± 0.12) | 4.18 (± 0.42) | 8.87 (± 3.04) | 3.12 (± 0.81) | 3.17 (± 1.14) |
| C16:1 | 17.76* (± 1.76) | 10.01* (± 3.17) | 35.63 (± 3.90) | 47.04 (± 1.82) | 41.27 (± 2.78) | 25.38 (± 7.47) | 23.94 (± 4.33) | 36.37 (± 2.07) |
| C18:1 | 16.37* (± 0.86) | 4.96* (± 0.15) | 31.95* (± 0.25) | 5.42* (± 0.25) | 20.15 (± 0.08) | 27.88 (± 0.64) | 28.86 (± 0.53) | 28.83 (± 0.19) |
| C20:1 | 24.05* (± 2.99) | 11.21* (± 0.61) | 45.56* (± 1.48) | 21.54* (± 3.51) | 40.60* (± 1.72) | 8.46* (± 1.93) | 43.64 (± 0.96) | 45.09 (± 0.83) |
| C22:1 | 0.00 (± 1.82) | 1.05 (± 1.50) | 0.51 (± 1.26) | 0.55 (± 0.82) | 20.12* (± 3.21) | 0.00* (± 0.19) | 4.91 (± 1.38) | 24.13 (2.22) |
| C24:1 | 0.31 (± 0.52) | 0.00 (± 1.58) | 8.36 (± 1.75) | 5.29 (± 4.18) | 9.42* (± 2.07) | 2.63* (± 1.14) | 8.10 (± 2.10) | 8.39 (± 1.70) |
| C26:1 | 36.45 (± 11.48) | 27.56 (± 1.86) | 59.32* (± 4.71) | 47.30* (± 3.37) | 70.80* (± 2.30) | 0.00* (± 0.00) | 59.41* (± 3.28) | 0.00* (± 0.00) |