TABLE 4.
Fatty acid profiles of yeast wild type and complement elo2/elo3 mutant strain
Values indicated are % of relative response units of total fatty acid methyl esters. p values were calculated using a two-tailed Student's t test, setting not detected fatty acid species to 0. The results of fatty acid extractions from two independent sets of cultures are shown. Fatty acyl species were identified by GC/MALDI mass spectroscopy as described under “Experimental Procedures.” ND means not detected.
| C14 | C15 | C16:1/16 | C18:1/18 | C20:1/20 | C22:1 | C22 | C24:1 | C24 | C26 | C26-OH | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| TgELO-B | 2.5 ± 1.2 | 4.4 ± 1.9 | 26.1a ± 3.8 | 36.3 ± 5.0 | 12.4b ± 3.2 | 14.8b ± 1.3 | 2.2 ± 1 | 1.6b ± 0.2 | ND/0.1 | ND | NDa |
| TgELO-C | 2.1 ± 0.6 | 3.6 ± 2.3 | 47.2 ± 3.9 | 45.0a ± 0.4 | 2.2 ± 1.6 | 0.2 / ND | ND | ND | ND | ND | NDa |
| ScELO3 | 2.5 ± 0.1 | 2.4 ± 1.5 | 40.6 ± 0.8 | 51.9 ± 1.8 | ND | ND | ND | ND | ND | 1.1 ± 0.6 | 1 ± 0.1 |
| WT | 3.1 ± 1.4 | 1.6 ± 1.5 | 47.2 ± 6.5 | 46.1 ± 2.2 | 0.5 ± 0.1 | 0.3 ± 0.2 | 0.5 ± 0.5 | ND | 0.1/ND | 0.6 ± 0,4 | 0.7/ND |
a p < 0.05 is only in comparison with the S. cerevisiae elo2/elo3 double mutant complemented with S. cerevisiae ELO3.
b p < 0.05 is in comparison with both S. cerevisiae WT and elo2/elo3 double mutant complemented with S. cerevisiae ELO3.