FIGURE 3.
Human recombinant Reg-1α increases number of PC12 and N2a cells with longer neurites. A, phase-contrast images of N2a cells in culture. Note the increase of neurite length 48 h after addition of 10−7 m Reg-1α in the medium (right panel) compared with untreated, control cells (left panel). Scale bars, 10 μm. B, quantification of the percentage of undifferentiated (white) and differentiated (i.e. neurite length is twice that of the cell body) (black) PC12 and N2a cells. Treatment with 10−7 m recombinant Reg-1α increases the number of cells with longer neurites (black) in comparison with untreated controls. Asterisks indicate significant differences from controls based on the Student's t test analysis (*, p < 0.05; **, p < 0.01).