TABLE 2.
Silencing of endogenous Reg-1α by siRNA decreases number of PC12 and N2a cells considered as differentiated
Quantification of the percentage of undifferentiated and differentiated (i.e. neurite length is twice that of the cell body) PC12 and N2a cells after down-regulation of endogenous Reg-1α by siRNA2. Data are expressed as mean ± S.E.
| PC12 cells |
N2a cells |
|||
|---|---|---|---|---|
| Control | siRNA | Control | siRNA | |
| Undifferentiated cells (%) | 88 ± 4.5 | 95 ± 9 | 91 ± 4.5 | 95 ± 2.3 |
| Differentiated cells (%) | 12 ± 3.8 | 5 ± 2a | 9 ± 2.2 | 5 ± 1.2a |
a Indicates significant differences from control cells (Student's t test, p < 0.05).