TABLE 3.

Effects of GFP-tagged Reg-1α overexpression on neurite outgrowth in PC12 and N2a cells and hippocampal neurons

Total neurite length per cell, longest neurite length, and number of neurites per cell were measured and are expressed as mean ± S.E.

	PC12 cells		N2a cells		Hippocampal neurons
	peGFP	Reg1-α-GFP	peGFP	Reg1-α-GFP	peGFP	Reg1-α-GFP
Total neurite length per cell (μm)	74.9 ± 15	128.5 ± 18a	27.6 ± 4	40.2 ± 3a
Longest neurite length per cell (μm)	47.8 ± 4	69.5 ± 10a	17.8 ± 1,6	26.8 ± 2b	68 ± 6	90.2 ± 5a
Neurites per cell	2.7 ± 0.6	3.1 ± 0.5	1.3 ± 0.1	1.5 ± 0.1	3.8 ± 0.1	3.7 ± 0.08

^a Indicates significant differences from control cells (Student's t test analysis, p < 0.05).

^b Indicates significant differences from control cells (Student's t test analysis, p < 0.01).