TABLE 4.

Effects of Reg-1α-pIRES2-eGFP and Reg-1αΔPS-pIRES2-eGFP mutant overexpression on neurite outgrowth of transfected and neighboring, untransfected PC12 cells

Total neurite length per cell, longest neurite length, and number of neurites per cell were measured and are expressed as mean ± S.E.

PC12 cells	pIRES2-eGFP	0–100 μm	100–200 μm	Reg-1α-pIRES2-eGFP	0–100 μm	100–200 μm	Reg-1αΔPSpIRES2-eGFP	0–100 μm	100–200 μm
Total neurite length per cell (μm)	40 ± 4.5	38.9 ± 2	38.1 ± 4.4	57 ± 3.7a	52.8 ± 3.1b	37.8 ± 2.3	37.1 ± 2.6	35.7 ± 2.3	34 ± 3.1
Longest neurite length per cell (μm)	26.4 ± 1.5	26.1 ± 0.8	25.2 ± 2.3	38.5 ± 1.8a	35.8 ± 1.6a	27.1 ± 1.5	25 ± 1.1	25.1 ± 1.4	22.6 ± 1.5
Neurites per cell	1.7 ± 0.1	1.7 ± 0.07	1.8 ± 0.1	1.7 ± 0.1	1.72 ± 0.08	1.8 ± 0.1	1.7 ± 0.1	1.73 ± 0.1	1.72 ± 0.1

^a Indicates significant differences from control cells transfected with pIRES2-eGFP vector alone (Student's t test, p < 0.001).

^b Indicates significant differences from control cells transfected with pIRES2-eGFP vector alone (Student's t test, p < 0.01).