FIGURE 5.

ARID2 is not required for BRG1-dependent repression of markers of fully mature osteocytes in pre-osteoblasts. A, total RNA was isolated from parental cells, the non-targeting control line, and BRG1-depleted and ARID2-depleted pre-osteoblasts, and qRT-PCR was carried out to assess the relative expression of Dmp1 and Phex. Shown are mean values relative to the parental cells with S.E. (n = 3). B, parental and ARID2-depleted pre-osteoblasts were analyzed by ChIP assay for association of the ATPase and ARID family subunits as well as selected transcriptional activators with the Dmp1 and Phex promoters. IP, immunoprecipitation.