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. 2011 Dec 23;287(7):4485–4491. doi: 10.1074/jbc.C111.291518

FIGURE 4.

FIGURE 4.

Depletion of cytoplasmic MTDH induces nuclear Rad51 accumulation, alters cell cycle profile, increases sensitivity to MMC and BIBF1120, and inhibits tumor proliferation in vivo. A, immunostaining of Rad51 in the nuclei was examined in control (left) or MTDH-depleted (center) Hec50 cells cultured in 10% FBS or control Hec50 cells cultured in 0.1% FBS (right). The arrow denotes mitotic cells. B, cell cycle profile was analyzed by flow cytometry after EdU pulse incorporation for 1 h in control or MTDH-depleted Hec50 cells cultured for 2 days in either 10% FBS or 0.1% FBS. C, colony formation of control or MTDH knockdown Hec50 cells was assessed after treatment with the indicated doses of MMC (ng/ml) for 1 day followed by culture in normal medium for 2 weeks. Results represent the average of three experiments. D, viability of control or MTDH-depleted cells in the presence of BIBF1120 for 3 days was detected by WST-1 assay. E, tumor volume was examined weekly in athymic mice injected with control or MTDH-depleted Hec50 cells (n = 4 animals per group). Error bars in C–E indicate S.D.