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. 2011 Dec 14;287(7):4562–4571. doi: 10.1074/jbc.M111.323113

FIGURE 3.

FIGURE 3.

Steady-state kinetics of holo-NR, Mo-heme fragment, and heme-FAD fragment under normal or viscous conditions. A, methyl viologen:nitrate assay of holo-NR using 0.4 mm methyl viologen in assay buffer (solid trace) or in assay buffer including 35% glycerol (dashed trace). B, methyl viologen:nitrate assay (details as above) of Mo-heme fragment in assay buffer (solid trace) or in assay buffer with 35% glycerol (dashed trace). C, NADH:cytochrome c activity assay of the heme-FAD fragment using 0.1 mm cytochrome c in assay buffer (solid trace) or in assay buffer including 35% glycerol (dashed trace). Error bars in all panels indicate S.D.