TABLE 4.
Catalytic efficiency values for the activation of the Pg variants by uPA in the absence or presence of Fg or Fn
Cofactor and Pg ratios were calculated as described in Table 3. Values represent the mean ± S.D. of at least 3 separate experiments.
| Cofactor | Catalytic efficiency | Cofactor ratio | Pg ratio | |
|---|---|---|---|---|
| (× 10−4) m−1s−1 | ||||
| None | 1.48 ± 0.72 | 1.0 | 1.0 | |
| Glu-Pg | Fg | 0.77 ± 0.30 | 0.5 | 1.0 |
| Fn | 0.57 ± 0.20 | 0.4 | 1.0 | |
| None | 14.85 ± 0.47 | 1.0 | 10.0 | |
| Lys-Pg | Fg | 10.24 ± 0.14 | 0.7 | 13.3 |
| Fn | 7.54 ± 0.54 | 0.5 | 13.2 | |
| None | 10.61 ± 0.58 | 1.0 | 7.2 | |
| Mini-Pg | Fg | 13.33 ± 1.46 | 1.3 | 17.3 |
| Fn | 10.96 ± 0.68 | 1.0 | 19.2 | |
| None | 6.06 ± 0.49 | 1.0 | 4.1 | |
| Micro-Pg | Fg | 3.63 ± 0.36 | 0.6 | 4.7 |
| Fn | 4.02 ± 0.77 | 0.7 | 7.1 | |