FIGURE 7.

BubR1 sumoylation is associated with its degradation. A, HeLa cells treated with nocodazole for various times as indicated after which equal amounts of cell lysates were blotted for BubR1, PARP-1, and β-actin. p89 represents a cleaved fragment of PARP-1. BubR1-D1 and BubR1-D2 denote two major BubR1 degradation products. B, HeLa cells treated with various agents as indicated for 24 h after which equal amounts of cell lysates were blotted for BubR1 and β-actin. MG132 and DEVD-CHO (20 μm) are proteasome and caspase 3 inhibitor, respectively. BubR1-D1 and BubR1-D2 denote two major degradation products of BubR1, respectively.