FIGURE 5.

N-cadherin redistribution in the in vitro implantation assay. A, Ishikawa cells were cultured with E2P4 (e), indicated concentrations of SAHA (f–h), or FA-5 (i–l). The media were replaced, and the green fluorescent JAR spheroids were then co-cultured for 24 h without stimulants. Co-cultured cells were fixed and stained with anti-N-cadherin mAb. Bar, 200 μm. B–D, total area intensities of N-cadherin signals were measured every 80 μm from the edge of the Ishikawa cell sheet adjacent to the spheroid. The in vitro implantation assay was performed for the indicated periods (B) or for 24 h (C and D), with or without 3 days of pretreatment with E2P4 or SAHA (C), or FA-5 (D). Each gray bar represents the mean ± S.E. of relative intensity obtained from three independent experiments. Asterisks show significant differences compared with the intensity in the area 400–480 μm from the edge of Ishikawa cell sheet adjacent to the spheroid (p < 0.05).