FIGURE 3.

Thermostability of NylC mutants. A, thermal transition curves of the various NylC mutant enzymes. CD measurements were performed at 220 nm from 25 to 95 °C (1 °C min⁻¹). The results are expressed as the mean residue molar ellipticity [θ]. Protein concentrations of 0.1 mg ml⁻¹ were used. deg, degree. B, the cumulative effects of amino acid mutations on the melting temperatures of denaturation (T_m) are shown.