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. 2011 Dec 19;287(7):4715–4725. doi: 10.1074/jbc.M111.323261

FIGURE 5.

FIGURE 5.

CREMα binds to the −127/−123 CRE site within the proximal IL17F promoter. A, naive human CD4+ T lymphocytes were isolated from four healthy individuals and cultured in the absence or presence of anti-CD3/anti-CD28 antibodies for 72 h. ChIP assays were performed in order to detect CREMα recruitment to the −127/−123 CRE site. Immunoprecipitated DNA was analyzed by real-time qPCR, amplifying a region that covers the CRE site within the proximal IL17F promoter. Ratios between anti-CREMα-immunoprecipitated and input DNA are shown. Dotted lines associate data between unstimulated and corresponding activated cells obtained from the same individual. Black bars represent the mean of the four experiments. B, CREMα ChIP was performed with total T lymphocytes from seven individually matched pairs of SLE patients and healthy controls. Immunoprecipitated DNA was analyzed by real-time qPCR. Ratios between anti-CREMα-immunoprecipitated and input DNA are shown. Dotted lines associate data between matched control/SLE pairs. Black bars, mean values. NS, not significant.