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Plasma half-life of scDb-IgBD and scFv-IgBD fusion proteins compared with unmodified proteins (scDb, scFv) and a chimeric IgG. scDb-IgBD (a) and scFv-IgBD (b) fusion proteins were injected intraperitoneally into CD1 mice (25 μg/animal), and serum concentrations of the antibody molecules were determined at different time points by ELISA. Data were normalized considering maximal concentration at the first time point (3 min).
