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UV-Vis spectra of SacD (A) and HPLC analysis of the hydroxylation of 3-Me-Tyr to 3-OH-5-Me-Tyr catalyzed by SacD (B). (i) Authentic standard of 3-Me-Tyr; (ii) authentic standard of 3-OH-5-Me-Tyr; (iii) negative control with boiled enzyme; (iv) reaction mixture; (●), 3-OH-5-Me-Tyr, (○), 3-Me-Tyr.
