FIGURE 6.

Suppression of IL-22 mRNA expression upon blockage of translation. Jurkat T cells were either kept as unstimulated control or stimulated with T (100 ng/ml)/A (10 μm) for 3 h. Where indicated, cells were preincubated for 1 h with CHX (10 μg/ml) before T/A addition. All cultures were adjusted to a final concentration of 0.1% DMSO (vehicle for T/A, CHX). After 4 h, IL-22 mRNA was determined by real time PCR analysis. IL-22 mRNA was normalized to that of GAPDH and is shown as mean fold-induction compared with unstimulated control ± S.D. (n = 6); **, p < 0.01 compared with unstimulated control; ##, p < 0.01 compared with T/A alone; raw data were analyzed by one-way ANOVA with post hoc Bonferroni correction.