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. 2011 Dec 17;287(7):4702–4714. doi: 10.1074/jbc.M111.305102

FIGURE 6.

FIGURE 6.

IRSp53 filopodium formation involves mDia1 and WAVE2. A, N1E115 cells were cotransfected with GFP-IRSp53, mCherry-Abp140p, and either mDia1 or nontargeting control siRNA, and time-lapse imaging of GFP-positive cells done at 24 h post-transfection. Bar, 10 μm. Magnified sections of transfected cells are shown in panels a and b with arrowheads indicating individual filopodia. B, N1E115 cells were cotransfected with GFP-IRSp53, mCherry-Abp140p, and either mDia2 or nontargeting control siRNA, and time-lapse imaging of GFP-positive cells done at 40 h post-transfection. Bar, 10 μm. Magnified sections of transfected cells are shown in panels a and b with arrowheads indicating individual filopodia. C, N1E115 cells were transfected with GFP-IRSp53, mCherry-Abp140p, and either WAVE2 or nontargeting control siRNA, and time-lapse imaging of GFP-positive cells done at 42 h post-transfection. Bar, 10 μm. Magnified sections of transfected cells are shown in panels a and b with arrowheads indicating individual filopodia. For fluorescence images, fluorescence intensity is represented using a pseudocolor scale (from highest to lowest intensity: white, red, orange, yellow, green, blue, purple, and black) that allows structures with relatively low fluorescence intensity such as filopodia to be easily visualized.