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Far Western (FW) analysis of IRS1 association with recombinant p85α-GST and endogenous p85α/β. Upper panels, representative blots of immunoprecipitation (IP) with indicated probe or antibodies. Lower panels, bar graph of densitometry quantitations of p85α-GST signals. PMA, phorbol myristate acetate; PY, antibody to phosphotyrosine. AU, arbitrary units.
