FIGURE 5.
Localization of RGS6 and different components of the GABABR-GIRK signaling axis in mouse cerebellum. A, genetic ablation of RGS6 does not change expression of GABABR2 or Gαi3 in cerebellum or cerebrum by Western blot. Actin serves as a loading control. B, co-immunostaining of RGS6 with Gβ5, GABABR2, GIRK1, GIRK2, and in cerebellar sections (5 μm) from WT mice reveals expression of all proteins in cerebellar granule layer (scale bar = 100 μm; white boxes, regions shown in enlarged merged images; white arrows, regions of granule cell specific protein enrichment).